Cryo-electron microscopy in structure determination
Current Research
Structural and functional characterization of DNA replication complexes
Elucidating the mechanisms regulating replication initiation in eukaryotes
Understanding the basis of replicative helicase loading and activation
Publications and News
Li, N., Gao, Y., Zhang, Y., Yu, D., Lin, W., Feng, J., Li, J., Xu, Z., Zhang, Y., Dang, S., Liu, Y., Zhou, K., Tye, B., Li, Q., Gao, N., Zhai, Y. (2024) Parental histone transfer caught at the replication fork. Nature https://doi.org/10.1038/s41586-024-07152-2
Li, J., Dong, J., Wang, W., Yu, D., Fan, X., Hui, Y., Lee, C., Lam, H., Alary, N., Yang, Y., Zhang, Y., Zhao, Q., Chen, C., Tye, B., Dang, S., and Zhai, Y. (2023) The Human Pre-replication Complex is an Open Complex. Cell 186, 98-111 https://doi.org/10.1016/j.cell.2022.12.008
Lin, J., Wu, Y., Tian, G., Yu, D., Yang, E., Lam, W., Liu, Z., Jing, Y., Dang, S., Bao, X., Wong J., Zhai, Y., & Li X. (2023) Menin 'reads' H3K79me2 mark in a nucleosomal context. Science 379, 717-723 https://doi.org/10.1126/science.adc9318
Xu, Z., Feng, J., Yu, D., Huo, Y., Ma, X., Lam, W., Liu, Z., Li, X., Ishibashi, T., Dang, S., Zhai, Y. (2023) Synergism between CMG Helicase and Leading Strand DNA Polymerase at Replication Fork. Nature Communications 14: 5849 https://doi.org/10.1038/s41467-023-41506-0
Cheng, J., Li, N., Huo, Y., Dang, S., Tye, B., Gao, N., and Zhai, Y. (2022) Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase Nature Communications 13:1396 https://doi.org/10.1038/s41467-022-29070-5
Lee, S., Cheung, M., Li, J., Zhao, Y., Lam, W., Ho, V., Rohs, R., Zhai, Y., Leung, D., Tye, B. (2021) Humanizing the Yeast Origin Recognition Complex. Nature Communications 12, 33 https://doi.org/10.1038/s41467-020-20277-y
Li, N., Lam, W., Zhai, Y., Cheng, J., Cheng, E., Zhao, Y., Gao, N. & Tye, B. (2018) Structure of the origin recognition complex bound to DNA replication origin. Nature 559, 217-222, https://www.nature.com/articles/s41586-018-0293-x
Zhai, Y. , Li, N., Jiang, H., Huang, X., Gao, N., Tye, B. (2017) Unique roles played by each of the non-identical MCM subunits in DNA Replication Licensing. Molecular Cell 67, 168-179. https://doi.org/10.1016/j.molcel.2017.06.016
Zhai, Y., Cheng, E., Wu, H., Li, N., Yung, P., Gao, N., and Tye, B. Open-ringed Structure of the Cdt1-MCM2-7 Complex as a Precursor of the MCM Double Hexamer. (2017) Nature Structural & Molecular Biology 24, 300-308 https://doi.org/10.1038/nsmb.3374
Li, N., Zhai, Y., Zhang, Y., Li, W., Yang, M., Lei, J., Tye, B., and Gao, N. (2015) Structure of the Eukaryotic Minichromosome Maintenance Complex at 3.8 Å. Nature, 524, 186–191 https://www.nature.com/articles/nature14685