Label-Free Optical Sensing and Characterization of Biomolecules by d8 or d10 Metal Complexes
Year: 2012 / 2011
Inventor: Professor Yam Wing Wah Vivian and others
The present invention provides a composition for detecting and/or characterizing a multiple-charged biomolecule comprising a charged d8 or d10 metal complex, wherein the metal complex electrostatically binds to the multiple-charged biomolecule to induce aggregation and self-assembly of the metal complex through metal . . . Metal interactions, [pi] . . . [pi] interactions, or a combination of both interactions. The present invention further provides assay methods and kits for label-free optical detection and/or characterization of biomolecules carrying multiple charges, e.g., single-stranded nucleic acids, polyaspartate, polyglutamate, using a composition comprising a charged d8 or d10 metal complex.